I have some exciting news!
Over the past week, I found over twenty Nylanderia parvula queens. One day I noticed two queens outside that were somewhat near each other, so I caught them both and put them in a test tube as an experiment to see if they were polygynous. Polygynous queens band together to build a nest and found a multi-queen colony. The first sign that they were polygynous was that when I moved them into a test tube, both queens immediately ran inside. To my surprise, the next day, the pair had a small brood pile! They are also very tolerant of each other, indicating that they might be polygynous.
A few days later, I looked again and saw that the brood pile had grown a lot. This means that both queens are likely mated. I checked some of my solo queens for comparison and noticed that their brood piles were smaller than what these two queens have.
